Journal: Molecular Medicine Reports

Article Title: Downregulation of inhibitor of apoptosis-stimulating protein of p53 inhibits proliferation and promotes apoptosis of gastric cancer cells

doi: 10.3892/mmr.2015.3587

Figure Lengend Snippet: iASPP expression is significantly downregulated in gastric cancer cells following infection with iASPP-siRNA-lentivirus, while the control siRNA had no effect on iASPP protein levels. (A) iASPP expression in MKN-45 cells was inhibited following iASPP-siRNA-lentivirus infection. (B) iASPP expression in SGC-7901 cells was inhibited following iASPP-siRNA-lentivirus infection. Values are expressed as the mean ± standard deviation; * P<0.05. iASPP, inhibitor of apoptosis-stimulating protein of p53; siRNA, small interfering RNA.

Article Snippet: Target cells were plated in six-well plates at 20–30% confluence and incubated for 12 h prior to the infections with iASPP-small interfering (si)RNA- or scrambled control-siRNA-expressing lentiviruses (Genepharma Co., Ltd.).

Techniques: Expressing, Infection, Standard Deviation, Small Interfering RNA

Journal: Frontiers in Oncology

Article Title: MicroRNA-483-5p Predicts Poor Prognosis and Promotes Cancer Metastasis by Targeting EGR3 in Nasopharyngeal Carcinoma

doi: 10.3389/fonc.2021.720835

Figure Lengend Snippet: EGR3 was a direct target of miR-483-5p in nasopharyngeal carcinoma (NPC) cell lines. (A) Wild-type (Wt) or mutant (Mt) target sequences of the EGR3 mRNA 3′-UTR. (B) The luciferase reporter assay was performed in 5-8F cells transfected with the psiCHECK luciferase reporter plasmid containing the Wt 3′-UTR of EGR3 , Mt 3′-UTR of EGR3 , a miR-483-5p mimic, and the negative control. (C) Western blot assay of the protein level of EGR3 in 5-8F cells after transfection with the miR-483-5p inhibitor. (D) Relative quantification of the mRNA expression of EGR3 by quantitative RT-PCR in 5-8F cells after transfection with the miR-483-5p inhibitor.* p < 0.05, *** p < 0.001.

Article Snippet: The miRNA inhibitor, scrambled negative control (NC) oligonucleotides, and EGR3 small interfering RNA (siRNA) were purchased from RiBoBio (Guangzhou, China).

Techniques: Mutagenesis, Luciferase, Reporter Assay, Transfection, Plasmid Preparation, Negative Control, Western Blot, Expressing, Quantitative RT-PCR

Journal: Frontiers in Oncology

Article Title: MicroRNA-483-5p Predicts Poor Prognosis and Promotes Cancer Metastasis by Targeting EGR3 in Nasopharyngeal Carcinoma

doi: 10.3389/fonc.2021.720835

Figure Lengend Snippet: EGR3 was decreased in nasopharyngeal carcinoma (NPC) clinical specimens, and its decrease could enhance NPC cell migration and invasion in vitro . (A) Relative expression of EGR3 mRNA by quantitative RT-PCR in NPC tissues compared with non-tumor tissues. (B) Relative expression of EGR3 mRNA by quantitative RT-PCR in 5-8F cells after transfection with siRNA. (C) Cell migration ability measured by the scratch wound healing assay. (D) Cell invasion ability measured by Boyden chamber assays with Matrigel. ** p < 0.01.

Article Snippet: The miRNA inhibitor, scrambled negative control (NC) oligonucleotides, and EGR3 small interfering RNA (siRNA) were purchased from RiBoBio (Guangzhou, China).

Techniques: Migration, In Vitro, Expressing, Quantitative RT-PCR, Transfection, Wound Healing Assay

Journal: Oncology Letters

Article Title: NCAPG upregulation mediated by four microRNAs combined with activation of the p53 signaling pathway is a predictor of poor prognosis in patients with breast cancer

doi: 10.3892/ol.2021.12585

Figure Lengend Snippet: NCAPG-knockdown decreases CDC25C expression and results in cell cycle arrest at the G 2 /M phase. (A) Effect of siRNA-mediated knockdown of NCAPG in the MCF-7 cell line. CDC25C (B) mRNA and (C) protein expression following knockdown of NCAPG in MCF-7 cells. Effects of (D) siRNA-NC, (E) siRNA-NCAPG1 and (F) siRNA-NCAPG2 on cell cycle distribution in MCF-7 cells. *P<0.05. NCAPG, non-SMC condensin I complex subunit G; CDC25C, cell division cyclin 25 homolog C; siRNA, small interfering RNA; NC, negative control.

Article Snippet: Small interfering (si)RNAs targeting NCAPG and a scrambled control siRNA used as the negative control (NC) were purchased from Guangzhou RiboBio Co., Ltd. NCAPG siRNAs and the NC (50 nM) were transfected using Lipofectamine ® 3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) into cells, according to the manufacturer's protocol.

Techniques: Knockdown, Expressing, Small Interfering RNA, Negative Control